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CC-5591 amc5 mt+ [87D3]

Name: CC-5591 amc5 mt+ [87D3]
SKU: CC-5591
Availability: InStock

$30.00

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and was created by mutagenesis of 3A+ (CC-5589) with a cassette containing the APHVIII transgene from pSL18. It is a mitochondrial complex I-deficient strain that harbors an insertional mutation in the NUOB10 gene encoding the NUOB10 / PDSW subunit of mitochondrial complex I. It is paromomycin resistant.

This strain should be maintained in low-medium light conditions (max. 50 Âµmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

CC-5591 amc5 mt+ [87D3]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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