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CC-5592 amc5; NUOB10 mt+ [amc5C2]

Name: CC-5592 amc5; NUOB10 mt+ [amc5C2]
SKU: CC-5592
Availability: InStock

$30.00

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10; ARG7

This is the amc5 strain complemented with the wild-type NUOB10 gene.

Mutagenesis of amc5 with cosmid 7D10 (Rochaix cosmid library) containing the wild-type NUOB10 gene and selected based on ARG7 marker in the cosmid. It is paromomycin resistant and an arginine prototroph.

This strain should be maintained in low-medium light conditions (max. 50 Âµmol.m-2.s-1).

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

CC-5592 amc5; NUOB10 mt+ [amc5C2]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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