* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5596 amc5; NUOB10(C79S
$30.00
Yes
From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020
Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C79S,C91S-FLAG; APHVII
This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C79S and C91S substitution.
Mutagenesis of amc5 with plasmid pRS426 containing the NUOB10 gene encoding the double C79S and C91S substitutions with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.
This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.