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CC-5599 amc1(27) mt-

Name: CC-5599 amc1(27) mt-
SKU: CC-5599
Availability: InStock

$30.00

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-1

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is a cross of amc1(4C10) with 4C- resulting in meiotic progeny that is arg- and hygromycin sensitive. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. APHVII transgene present in an unknown location. Originally isolated as a mitochondrial complex I-deficient strain but now the phenotype has reverted.

This strain should be maintained in low-medium light conditions (max. 50 Âµmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin sensitive.

Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

CC-5599 amc1(27) mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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