CC-5608 amc11 mt- [10G11

$30.00

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2;APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

  • Locus:
  • Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.<br><br><br>Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:
  • Mutation Information:
  • Predicted Mutation Effect:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2;APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.