CC-5609 amc1-2; AMC11-A mt- [amc11C2

$30.00

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with cosmid 1H10 (Rochaix cosmid library) containing the full-length wild-type AMC11 (Cre16.g688900) and CYG56 (Cre16.g688901) genes and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).

  • Locus:
  • Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:
  • Mutation Information:
  • Predicted Mutation Effect:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with cosmid 1H10 (Rochaix cosmid library) containing the full-length wild-type AMC11 (Cre16.g688900) and CYG56 (Cre16.g688901) genes and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).