CC-5631 amc7 mt+ [111A4+]
$30.00
From Rosario Barbieri, Patrice P. Hamel lab, The Ohio State University, February 2020
Genotype: mt+; arg7-8; APHVIII
This strain was created by mutagenesis of 3A+ with a cassette containing the APHVII transgene from pSL18.
It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain in paromomycin resistant.
Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.