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CC-5633 amc11(70) mt+

Name: CC-5633 amc11(70) mt+
SKU: CC-5633
Availability: InStock

$30.00

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-2; APHVII

This mitochondrial complex I-deficient strain is a genetic cross of amc11(10G11) with a 137c mt+ strain (1′) from Dr. Claire Remacle (University of Liege) selected for arg+, hygromycin B resistant meiotic prorgeny. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is hygromycin B resistant and an arginine prototroph.

Maintain in medium light conditions (max. 50 Âµmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

Locus:
AMC1
Chromosome:
16
Mutation Information:
Predicted Mutation Effect:

CC-5633 amc11(70) mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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