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CC-5635 amc11 arg9-2 #90 mt+

Name: CC-5635 amc11 arg9-2 #90 mt+
SKU: CC-5635
Availability: InStock

$30.00

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-2; APHVII; arg9-2

This mitochondrial complex I-deficient strain is a genetic cross of amc11(70) with a complex I proficient derivative of arg9-2 (Hamel strain 141) to select for arg-, hygromycin B resistant meiotic progeny. The APHVII transgene disrupts Cre16.g688900 in exon 2.

Maintain in medium light conditions (max. 50 Âµmol.m-2.s-1) and not in the dark as it is respiratory-deficient. Media should contain arginine.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

Locus:
AMC1
Chromosome:
16
Mutation Information:
Predicted Mutation Effect:

CC-5635 amc11 arg9-2 #90 mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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