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CC-5639 RR6-∆ChR1+2 [PH012]

Name: CC-5639 RR6-∆ChR1+2 [PH012]
SKU: CC-5639
Availability: InStock

$30.00

Deposited by Irina Sizova, Peter Hegemann lab, Humboldt University of Berlin, September 2020

This is a ChR1-ChR2 disruption strain, generated with CRISPR/Cas9.

Background strain: CC-5637 RR6-∆ChR1 mt- [PH006]
Nuclease: ChR1-ZFN & ChR2-ZFN
Target gene: ChR1, Cre14.g611300
ChR2, Cre02.g085257
Target sequence: (ChR1) ZFN-L: CCCTCCGCC
ZFN-R: GCCGGCGGC

Target sequence: (ChR2) ZFN-L: CCACACCGTGCC
ZFN-R: CCGGTGTCGCCA

This strain was published in Greiner et al 2017.

Overview of all strains from the Hegemann lab
http://www.chlamy.de/strains

Visit www.chlamy.de for more info or contact CRISPR@chlamy.de

Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell. 2017 Oct;29(10):2498-2518. doi: 10.1105/tpc.17.00659. Epub 2017 Oct 4. PMID: 28978758; PMCID: PMC5774583.

CC-5639 RR6-∆ChR1+2 [PH012]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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