CC-5654 Cre10.g430350-Venus3xFLAG-W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A mt-

$30.00

From Moritz Meyer, Martin Jonikas lab, Princeton University, August 2020

This strain was created by transforming CMJ030 with pMJ509 and is paromomycin resistant.

pMJ509: pLM005-Cre10.g430350[W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A]-Venus-3xFLAG

Previously synthesized pMJ507 was modified by side-directed mutagenesis. All four triplet pairs coding for a Tryptophan-Arginine dipeptide were mutagenized to code for a double Alanine (W47A/R48A/W51A/R52A/W110A/R111A/W148A/R149A).