CC-5661 l1a-2 mt-

$30.00

From Alfredo Kono, Martin Spalding lab, Iowa State University, July 2020

This double mutant is the product of a cross of LMJ.RY0402.191570 mt- (LCI1 mutant) with lcia63 mt+ CC‐5066 (LCIA mutant).

Mutant alleles: LCIA, NAR 1.2, Cre06.g309000.t1.1, chromosome_6:8604782..8607670 reverse; LCI1, Cre03.g162800.t1.1, chromosome_3:2885264..2887871 reverse


Kono A, Spalding MH. LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO2 uptake under low CO2. Plant J. 2020 Jun;102(6):1127-1141. doi: 10.1111/tpj.14761. Epub 2020 Apr 27. PMID: 32248584.

Li X, Zhang R, Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC. An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii. Plant Cell. 2016 Feb;28(2):367-87. doi: 10.1105/tpc.15.00465. Epub 2016 Jan 13. PMID: 26764374; PMCID: PMC4790863.

Wang Y, Spalding MH. Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii. Plant Physiol. 2014 Dec;166(4):2040-50. doi: 10.1104/pp.114.248294. Epub 2014 Oct 21. PMID: 25336519; PMCID: PMC4256846.


  • Locus:
  • LCIA/NAR 1.2, LCI1
  • Chromosome:
  • 6,3
  • Mutation Information:
  • Predicted Mutation Effect:

Kono A, Spalding MH. LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO2 uptake under low CO2. Plant J. 2020 Jun;102(6):1127-1141. doi: 10.1111/tpj.14761. Epub 2020 Apr 27. PMID: 32248584.

Li X, Zhang R, Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC. An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii. Plant Cell. 2016 Feb;28(2):367-87. doi: 10.1105/tpc.15.00465. Epub 2016 Jan 13. PMID: 26764374; PMCID: PMC4790863.

Wang Y, Spalding MH. Acclimation to very low CO2: contribution of limiting CO2 inducible proteins, LCIB and LCIA, to inorganic carbon uptake in Chlamydomonas reinhardtii. Plant Physiol. 2014 Dec;166(4):2040-50. doi: 10.1104/pp.114.248294. Epub 2014 Oct 21. PMID: 25336519; PMCID: PMC4256846.