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CC-5664 ift81-1 IFT81HA mt+

Name: CC-5664 ift81-1 IFT81HA mt+
SKU: CC-5664
Availability: InStock

$30.00

From George B. Witman, University of Massachusetts Medical School, August 2020

In this strain, IFT81-HA is incorporated into the IFT-B complex and localizes normally in cell bodies and flagella. The cells appear to be fully rescued for motility and flagellar length.

It was created by insertional mutagenesis of the parent strain with a fragment conferring resistance to hygromycin B (aph7â€) to generate the null mutant ift81-1, followed by rescue with a DNA fragment encoding IFT81 with a 3xhemagglutinin (HA) tag at its C-terminal end and containing a paromomycin-resistance gene as a selectable marker.

Mutant allele: ift81-1, chromosome_17:3362408-3368081

Kubo T, Brown JM, Bellve K, Craige B, Craft JM, Fogarty K, Lechtreck KF, Witman GB. Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. J Cell Sci. 2016 May 15;129(10):2106-19. doi: 10.1242/jcs.187120. Epub 2016 Apr 11. PMID: 27068536; PMCID: PMC5506485.

Locus:
IFT81
Chromosome:
17
Mutation Information:
Predicted Mutation Effect:

CC-5664 ift81-1 IFT81HA mt+

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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