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CC-5771 RMC3 mt-

Name: CC-5771 RMC3 mt-
SKU: CC-5771
Availability: InStock

$30.00

From Anthony Berndt, Stephen Mayfield lab, University of California-San Diego, November 2021

ER-Golgi retained SARS-CoV-2 Spike RBD fused to mClover fluorescent protein.

Plasmid insertion makes strain Zeocin and Hygromycin resistant. RBD::mClover fusion protein is directed for secretion using the PHC2 secretion signal peptide and retained in the ER-Golgi network with a C-terminal KDEL motif. Expression may mildly attenuate over several months on non-selective media but can be restored by re-selection on 30 µg/mL Hygromycin B on regular tap plates.

Berndt AJ, Smalley TN, Ren B, Simkovsky R, Badary A, Sproles AE, Fields FJ, Torres-Tiji Y, Heredia V, Mayfield SP. Recombinant production of a functional SARS-CoV-2 spike receptor binding domain in the green algae Chlamydomonas reinhardtii. PLoS One. 2021 Nov 18;16(11):e0257089. doi: 10.1371/journal.pone.0257089. PMID: 34793485; PMCID: PMC8601568.

CC-5771 RMC3 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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