* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-5861 ift54-2::mNG-IFT54 mt-

Name: CC-5861 ift54-2::mNG-IFT54 mt-
SKU: 87603
Availability: InStock

$30.00

From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022

The strain allows imaging of IFT via the very bright IFT54-mNG signal.

Wingfield JL, Mengoni I, Bomberger H, Jiang YY, Walsh JD, Brown JM, Picariello T, Cochran DA, Zhu B, Pan J, Eggenschwiler J, Gaertig J, Witman GB, Kner P, Lechtreck K. IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife. 2017 May 31;6:e26609. doi: 10.7554/eLife.26609. PMID: 28562242; PMCID: PMC5451262.

Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.

Locus:
IFT54
Chromosome:
11

CC-5861 ift54-2::mNG-IFT54 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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