CC-5862 ift140-1::IFT140-sfGFP ift54-2::mS-IFT54 mt?
$30.00
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating ift140-1::IFT140-sfGFP and ift54-2::mScarlet-IFT54. It facilitates simultaneous 2-color imaging of the IFT-A and IFT-B subcomplexes.
Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB. A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J Cell Sci. 2019 Feb 11;132(3):jcs220749. doi: 10.1242/jcs.220749. PMID: 30659111; PMCID: PMC6382014.
Wingfield JL, Mengoni I, Bomberger H, Jiang YY, Walsh JD, Brown JM, Picariello T, Cochran DA, Zhu B, Pan J, Eggenschwiler J, Gaertig J, Witman GB, Kner P, Lechtreck K. IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife. 2017 May 31;6:e26609. doi: 10.7554/eLife.26609. PMID: 28562242; PMCID: PMC5451262.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.