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CC-5901 pf2; PF2-GcAMP6

Name: CC-5901 pf2; PF2-GcAMP6
SKU: CC-5901
Availability: InStock

$30.00

From Adrian Nievergelt, Pigino Group, Max-Planck Institute for Cell and Molecular Biology-Dresden, August 2022

CC-513 pf2 mt- rescued with a pf2 gene fused to a c-terminal GcAMP6 tag under the control of it’s endogenous 5’ and 3’ UTRs and promoter. Insertional co-transformation with pSI103.

This strain expresses a free calcium reporter which is fused to DRC4 (pf2). The strain will show bright green flashes in calcium abundant media such as TAP, often correlated with motion of or touch of the flagella. Resistant to paromomycin.

Nievergelt AP, Zykov I, Diener D, Chhatre A, Buchholz TO, Delling M, Diez S, Jug F, Štěpánek L, Pigino G. Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport. Curr Biol. 2022 Sep 26;32(18):4071-4078.e4. doi: 10.1016/j.cub.2022.07.033. Epub 2022 Aug 3. PMID: 35926510; PMCID: PMC9521741.

Locus:
PF2
Chromosome:
11
Mutation Information:
Predicted Mutation Effect:

CC-5901 pf2; PF2-GcAMP6

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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