* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5906 fap70::aphVIII-D1 mt+
$30.00
From George Witman, University of Massachusetts Medical School, August 2022
This strain was generated by the TIM method, a targeted insertional mutagenesis approach utilizing CRISPR/Cas9 and is paromomycin resistant.
It is from the experiment of Row 14 repeat 2 in Table 1 of Picariello, Hou et al., (2020) using donor DNA containing the paromomycin-resistant cassette and 50-bp homology arms.
Picariello T, Hou Y, Kubo T, McNeill NA, Yanagisawa HA, Oda T, Witman GB. TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii. PLoS One. 2020 May 13;15(5):e0232594. doi: 10.1371/journal.pone.0232594. PMID: 32401787; PMCID: PMC7219734.