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CC-5909 ac17; NIT2; atg17::aphVIII mt- [10-2]

Name: CC-5909 ac17; NIT2; atg17::aphVIII mt- [10-2]
SKU: CC-5909
Availability: InStock

$30.00

From Susan Dutcher, Washington University in St. Louis, September 2022

Strain 6B10 (background CC-124) containing an insertion of aphviii in the ATG11 gene (Lin, Cliften & Dutcher, 2018) was crossed to a strain of mixed background with genotype ac17; NIT2; ery1.

This strain fails to grow in medium lacking acetate. It also does not grow in minimal (M) medium and is resistant to paromomycin. The ATG17 gene was previously annotated and published as ATG11.

Payne, Z.L., Penny, G.M., Turner, T.N., & Dutcher, S.K. Detection of Translocations Induced by Site-Directed CRISPR/Cas9 Mutagenesis Enabled By Long-Read Genome Assembly of Chlamydomonas reinhardttii – In revision, main manuscript

Lin H, Cliften PF, Dutcher SK. MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 2018 Dec;178(4):1436-1447. doi: 10.1104/pp.18.00474. Epub 2018 Sep 11. PMID: 30206105; PMCID: PMC6288735.

Locus:
ac17 [PDH2]
Chromosome:
3
Mutation Information:
Predicted Mutation Effect:

CC-5909 ac17; NIT2; atg17::aphVIII mt- [10-2]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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