CC-5914 gspk (3’UTR) mt+

$30.00

From Peeyush Ranjan, Snell lab, University of Maryland College Park, September 2022

This strain has a mutation in the 3’UTR region. It was created by crossing the gspk CLiP mutant LMJ.RY0402.039382 (mt-) with wild type 21gr (mt+) cells. Selection was by antibiotic resistance (aphVIII) and colony PCR using the CHELEX method.


Awasthi M, Ranjan P, Kelterborn S, Hegemann P, Snell WJ. A cytoplasmic protein kinase couples engagement of Chlamydomonas ciliary receptors to cAMP-dependent cellular responses. J Cell Sci. 2022 May 15;135(10):jcs259814. doi: 10.1242/jcs.259814. Epub 2022 May 23. PMID: 35502650; PMCID: PMC9189427.