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CC-5937 psaA-3::aadA knockout [HNT6]

Name: CC-5937 psaA-3::aadA knockout [HNT6]
SKU: CC-5937
Availability: InStock

$30.00

From Saul Purton, University College London, August 2023

Origin: psaA-3::aadA knockout in which part of the psaA-3 coding region and 3’ UTR is replaced with the aadA cassette through chloroplast transformation. Background strain is CC-1690.

Culture maintenance: Acetate-requiring, light-sensitive (ΔPSI mutant)

Comment: This strain was created to serve as a recipient for chloroplast transformation where selection is based on restoration of light-insensitivity (and phototrophy) using the WT psaA-3 gene as the marker.

Taunt HN, Jackson HO, Gunnarsson ÍN, Pervaiz R, Purton S. Accelerating Chloroplast Engineering: A New System for Rapid Generation of Marker-Free Transplastomic Lines of Chlamydomonas reinhardtii. Microorganisms. 2023 Jul 31;11(8):1967. doi: 10.3390/microorganisms11081967. PMID: 37630526; PMCID: PMC10457852.

CC-5937 psaA-3::aadA knockout [HNT6]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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