* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-5991 oda3; oda6::IC2-NG ift20::IFT20-mC mt- [25B]
$30.00
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2023
This strain was obtained by mating oda3 oda6::IC2-NG and ift20::IFT20-mCherry. The outer dynein arms (ODAs) are tagged on the essential IC2 subunits with mNeonGreen and IFT is visible by IFT20-mCherry expression. ODAs do not assemble in cilia due to the absence of the docking complex in oda3. This allows imaging of ODA transport and IFT.
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Dai J, Barbieri F, Mitchell DR, Lechtreck KF. In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties. Mol Biol Cell. 2018 Oct 15;29(21):2553-2565. doi: 10.1091/mbc.E18-05-0291. Epub 2018 Aug 22. PMID: 30133350; PMCID: PMC6254574.