CC-6011 oda2-t tpg1 [#2]
$30.00
From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023
Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC; reduced axonemal tubulin polyglutamylation; mixed population of immotile cells and cells showing intermittent beating or swimming
This mutant was obtained by crossing oda2-t (strain 12c, CC-6009) and tpg1 (CC-5245).
Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R. Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 2010 Mar 9;20(5):441-5. doi: 10.1016/j.cub.2009.12.058. Epub 2010 Feb 25. PMID: 20188560.
Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.