CC-6013 mNeonGreen-IFT46-mScarletI

$30.00

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M

Origin: CRISPR mediated endogenous n-terminal mNeonGreen with introns + c-terminal mScarlet-I knock-in

Proof of concept strain for serially tagging the same gene on both termini. IFT content in this strain is reduced in quantity but present. Resistant to Paromomycin and Blasticidin S.

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.

  • Locus:
  • FT46 (Cre05.g241637_4532)
  • Chromosome:
  • 5
  • Mutation Information:
  • Predicted Mutation Effect:

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.