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CC-6016 mNeonGreen-TuA1 drc4::AphVIII

Name: CC-6016 mNeonGreen-TuA1 drc4::AphVIII
SKU: CC-6016
Availability: InStock

$30.00

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M

Origin: Co-targeted CRISPR mediated endogenous n-terminal mNeonGreen with introns + DRC4::AphVIII insertion

Proof of concept strain for cotargeted genome editing with region replacement by multiple guide RNAs. The expression of fluorescent tubulin is visible in a confocal microscope, but very expression is unusually low. Resistant to Paromomycin.

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.

Locus:
alpha tubulin, TUA1 (Cre03.g190950_4532)
Chromosome:
3
Mutation Information:
Predicted Mutation Effect:

CC-6016 mNeonGreen-TuA1 drc4::AphVIII

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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