CC-6019 FAP256-mNG ift46::NIT IFT46-mApple

$30.00

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M/CC-4375

Origin: Cross between CC-6017 and CC-6018

This strain has bright green fluorescent ciliary tips as well as zones of green fluorescence clost to the ciliary base and red fluorescent IFT. Resistant to Nourseothricin and Paromomycin.

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.

  • Locus:
  • FAP256 (Cre10.g431150_4532), IFT46 (Cre05.g241637_4532
  • Chromosome:
  • 10, 5
  • Mutation Information:
  • Predicted Mutation Effect:

Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.