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CC-6072 pf23-2 mt-

Name: CC-6072 pf23-2 mt-
SKU: CC-6072
Availability: InStock

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf23::aphVII; ac17; atg17::aphIII

Strain CC-5909 was transformed with aphVII construct targeted to exon 1 of the PF23 gene using CRISPR/Cas9.

This strain is a null allele of PF23. It lacks cilia under vegetative conditions and has extremetly short, stubby cilia as gametes. It does not grow in medium lacking acetate and is resistant to hygromycin and paromomycin.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Payne ZL, Penny GM, Turner TN, Dutcher SK. A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis. Plant Commun. 2023 Mar 13;4(2):100493. doi: 10.1016/j.xplc.2022.100493. Epub 2022 Nov 17. PMID: 36397679; PMCID: PMC10030371.

Lin H, Cliften PF, Dutcher SK. MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 2018 Dec;178(4):1436-1447. doi: 10.1104/pp.18.00474. Epub 2018 Sep 11. PMID: 30206105; PMCID: PMC6288735.

Locus:
PF23, AC17, ATG17
Chromosome:
11, 3, 16
Mutation Information:
Predicted Mutation Effect:

CC-6072 pf23-2 mt-

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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