CC-613 pf14 mt-
$30.00
From Ursula Goodenough, Harvard University, June 1978
Phenotype: impaired motility
This mutant is easy to score, and is an excellent marker for the right arm of linkage group VI.
The pf14 mutants lack radial spokes and are missing a set of 17 spoke-associated polypeptides. Huang and colleagues in Luck’s laboratory used dikaryon and revertant analysis to identify one of these as a probable gene product. The gene was subsequently cloned and sequenced in Rosenbaum’s laboratory, confirming identity of the gene product as radial spoke protein 3.
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85
Luck DJ, Piperno G, Ramanis Z, Huang B (1977) Flagellar mutants of Chlamydomonas: studies of radial spoke-defective strains by dikaryon and revertant analysis. Proc Natl Acad Sci USA 74:3456-3460
Diener DR, Ang LH, Rosenbaum JL (1993) Assembly of flagellar radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3. J Cell Biol 123:183-190
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM, Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB (2006) Radial spoke proteins of Chlamydomonas flagella. J Cell Sci 119(Pt 6):1165-1174