CC-6196 402_pJPSHx1PHL7
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Chlamydomonas pacifica CC-5697, transformed with the vector pJPSHx1 PHL7. This strain is resistant to hygromycin due to the incorporation of the aph7” gene and expresses the plastic-degrading enzyme PHL7, which is directed to the secretory pathway via the SP7 signal peptide.
The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.
For further details on the background of Chlamydomonas pacifica CC-5697, including its extremophilic properties and potential for biotechnology applications, please refer to the publication below.
Dataset info: https://doi.org/10.5281/zenodo.14020362
Dutra Molino JV, Oliver A, Sethuram H, Kang K, Saucedo B, Diaz CJ, Gupta A, Jen LJ, Torres-Tiji Y, Hidasi N, Badary A. Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host. bioRxiv. 2024:2024-09.