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CC-6199 403_pJP32PHL7

Name: CC-6199 403_pJP32PHL7
SKU: CC-6199
Availability: InStock

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas pacifica CC-5699, transformed with the vector pJP32PHL7. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the plastic degrading enzyme PHL7, which is directed to the secretory pathway via the SP7 signal peptide.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.

For further details on the background of Chlamydomonas pacifica CC-5697, including its extremophilic properties and potential for biotechnology applications, please refer to the publication below.

Dataset info: https://doi.org/10.5281/zenodo.14020362

Dutra Molino JV, Oliver A, Sethuram H, Kang K, Saucedo B, Diaz CJ, Gupta A, Jen LJ, Torres-Tiji Y, Hidasi N, Badary A. Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host. bioRxiv. 2024:2024-09.

CC-6199 403_pJP32PHL7

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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