CC-6201 cc1690_pJP32mCherry
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Chlamydomonas reinhardtii CC-1690, transformed with the vector pJP32mCherry. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene. It expresses the fluorescent protein mCherry, which is directed to the secretory pathway via the SP7 signal peptide.
The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.
Dataset info: https://doi.org/10.5281/zenodo.14020362
Molino JV, de Carvalho JC, Mayfield SP. Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS One. 2018 Feb 6;13(2):e0192433.