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CC-6202 cc1690_pJP32mCherry_His

Name: CC-6202 cc1690_pJP32mCherry_His
SKU: CC-6202
Availability: InStock

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas reinhardtii CC-1690, transformed with the vector pJP32mCherry_His. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene. It expresses the fluorescent protein mCherry plus 6xHis tag, which is directed to the secretory pathway via the SP7 signal peptide.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.

Dataset info: https://doi.org/10.5281/zenodo.14020362

Kang, K., Do Espirito Santo, É., Diaz, C. J., Oliver, A., Saxton, L., May, L., Mayfield, S., & Dutra Molino, J. V. (2024). Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production.
https://doi.org/10.1101/2024.10.25.618925

CC-6202 cc1690_pJP32mCherry_His

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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