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CC-6203 cc1690_pJP32PHL7_G12

Name: CC-6203 cc1690_pJP32PHL7_G12
SKU: CC-6203
Availability: InStock

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas reinhardtii CC-1690, transformed with the vector pJP32PHL7. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the plastic degrading enzyme PHL7, which is directed to the secretory pathway via the SP7 signal peptide.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.

For further details on Chlamydomonas reinhardtii CC-1690 pJP32PHL7, please refer to the publication below.

Dataset info: https://doi.org/10.5281/zenodo.14020362

Molino JV, Saucedo B, Kang K, Walsh C, Diaz CJ, Tessman M, Mayfield SP. Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii. bioRxiv. 2024:2024-10.

CC-6203 cc1690_pJP32PHL7_G12

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

Any opinions, findings, and conclusions or recommendations expressed on this website are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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