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CC-6205 cc1690_pJPW4_mCherry

Name: CC-6205 cc1690_pJPW4_mCherry
SKU: CC-6205
Availability: InStock

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas reinhardtii CC-1690, transformed with the vector pJPW4_mCherry. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the fluorescent protein mCherry, which is directed to the cell wall via the SP7 signal peptide and linkage to a GP1, hydroxyproline-rich protein hypothesized to be attached to the cell wall by non-covalent interactions. A pH-sensitive intein is placed between mCherry and the GP1 protein and appears to release mCherry from the cell wall at lower pH <6.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.

Dataset info: https://doi.org/10.5281/zenodo.14020362

CC-6205 cc1690_pJPW4_mCherry

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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