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CC-6209 Ci_pJPW2_mCherry

Name: CC-6209 Ci_pJPW2_mCherry
SKU: CC-6209
Availability: InStock

$30.00

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas incerta CC-3871, transformed with the vector pJPW2_mCherry. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the fluorescent protein mCherry, which is directed to the cell wall via the SP7 signal peptide and linkage to a GP1, hydroxyproline-rich protein hypothesized to be attached to the cell wall by non-covalent interactions.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source.

Dataset info: https://doi.org/10.5281/zenodo.14020362

Kang, K., Do Espirito Santo, É., Diaz, C. J., Oliver, A., Saxton, L., May, L., Mayfield, S., & Dutra Molino, J. V. (2024). Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production.
https://doi.org/10.1101/2024.10.25.618925

Molino JV, Carpine R, Gademann K, Mayfield S, Sieber S. Development of a cell surface display system in Chlamydomonas reinhardtii. Algal Research. 2022 Jan 1;61:102570.

CC-6209 Ci_pJPW2_mCherry

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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