* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6223 fmg1b fmg1a ift46::NIT IFT46-mNG

Name: CC-6223 fmg1b fmg1a ift46::NIT IFT46-mNG
SKU: CC-6223
Availability: InStock

$30.00

From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025

Background: CC-124/CC-125
Origin: Cross of CC-6221 and CC-5900 to allow for observation of intraflagellar transport and gliding activity in the absence of the ciliary coat proteins FMG1A and FMG1B.
Description: This strain lacks both A and B isoforms of FMG1. The strain has altered ciliary adhesion. Intraflagellar transport is labelled for green fluorescence. Nourseothricin, Spectinomycin, and Paromomycin resistant.
Locus: FMG1B (Cre09.g392867_4532, Chromosome 9), FMG1A (Cre18.g750047_4532, Chromosome 9), IFT46 (Cre05.g241637_4532, Chromosome 5)

Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.

CC-6223 fmg1b fmg1a ift46::NIT IFT46-mNG

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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