* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

CC-6295 ΔHLM4; ΔDMC5; ΔMUT9 mt+ [Δ459]

Name: CC-6295 ΔHLM4; ΔDMC5; ΔMUT9 mt+ [Δ459]
SKU: CC-6295
Availability: InStock

$30.00

From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025

Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase

Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.

CC-6295 ΔHLM4; ΔDMC5; ΔMUT9 mt+ [Δ459]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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