* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-67 er-u-37 mt-
$30.00
Karen VanWinkle-Swift, Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (erythromycin)
Isolated by Karen VanWinkle-Swift. This is her original isolate.
This is a good chloroplast marker for erythromycin resistance, and has been used in many crosses. It maps to the center of the 23S rRNA gene, proximal to the intron, and is a G -> A base change in the 23S rRNA at the position equivalent to E. coli 2057.
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292