* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-87 nr-u-2-1 mt-
$30.00
Boynton-Gillham laboratory, Duke University
Phenotype: antibiotic resistant (kanamycin or neamine)
Product from a cross of CC-86 nr-u-2-1 mt+ x arg2 mt-. This stock is not arginine-requiring.
This is a useful chloroplast marker, mapping to the 3′ end of the chloroplast 16S ribosomal RNA. The mutation is an A>G change at nucleotide 1340. Although isolated initially as a neamine-resistant strain, it is also resistant to kanamycin, a more readily available antibiotic.
Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292