CC-942 ery1b mt-
$30.00
From Maureen Hanson, University of Virginia, February 1980
Phenotype: antibiotic resistant (erythromycin)
The ery1b mutation was isolated in Bogorad’s laboratory in the 137c background, induced by mutagenesis with ethyl methanesulfonate. This strain has been used in many crosses by the Chlamydomonas Center and has been well-behaved, easy to score.
The ery1 mutations were shown by Davidson et al. (1974) to alter a protein of the large subunit of the chloroplast ribosome, subsequently identified by Bowers et al. as the product of the RPL4 gene. The ery1b allele results in an L4 protein that is about 30% smaller than the comparable wild-type protein.
Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119-129
Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353
Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119-129
Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353