Hydrogen evolution
$5.00 – $35.00
Chlamydomonas, like a number of other kinds of algae, has a reversible hydrogenase that can reduce protons to molecular hydrogen. Melis et al. (Plant Physiology 122, 127-135, 2000) have shown that this activity is inducible in photosynthetically growing cells on deprivation of oxygen and sulfur. This process has potential economic importance as a source of hydrogen for fuel, especially if the native hydrogenase can be modified genetically to reduce its sensitivity to oxygen.
Hydrogen project kit for one student:
- Two Chlamydomonas cultures (CC-125) in liquid and one on agar plus stock solutions for 5 liters each of complete and sulfur-deficient media
- Copies of the Melis et al. paper and a review paper, plastic tubing, connectors and instructions
You will also need a fluorescent lamp and two 750 ml or 1-liter bottles of commercial spring water, preferably the kind with a pull-up drinking spout.
Hydrogen evolution supplement: Cultures of CC-125 (2 in liquid, 1 on agar) plus stock solutions for 5 liters each of complete and sulfur-deficient media. **This is reserved for users that have ordered the Hydrogen Evolution Kit.
10 ml liquid culture: 10 ml liquid culture of Chlamydomonas reinhardtii CC-125 for additional experiments. **This is reserved for users that have ordered the Hydrogen Evolution Kit.