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P-104 cpDNA EcoRI 36/37 (0.9 kb) spr-u-1-6-2

Name: P-104 cpDNA EcoRI 36/37 (0.9 kb) spr-u-1-6-2
SKU: P-104
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1986

Chloroplast DNA EcoRI 36/37 (0.9 kb) in pUC8; contains 16S rRNA gene (3′ end) from spectinomycin resistant mutant CC-110 spr-u-1-6-2

host strain: JM83
amp resistant

More information

Strain: CC-110 spr-u-1-6-2 mt+

Insert: EcoRI 36/37 (0.9 kb)

Genes present: 16S rRNA (3′ end)

Vector: pUC8

Bacterial host strain: JM83

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1986

Contains A->G change at bp 1123 in C. reinhardtii 16S rRNA gene, causing loss of an Aat II site and conferring high level spectinomycin resistance.
Harris et al., Genetics 123, 281 (1989).

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292

P-104 cpDNA EcoRI 36/37 (0.9 kb) spr-u-1-6-2

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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