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P-198 cpDNA EcoRI-BamHI (2.0 kb) sr-u-2-60

Name: P-198 cpDNA EcoRI-BamHI (2.0 kb) sr-u-2-60
SKU: P-198
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1989

Chloroplast DNA EcoRI-BamHI (2.0 kb) in pUC8; 5′ end of 16S rRNA gene; contains streptomycin resistance mutation sr-u-2-60, from CC-118

host strain: TG-1
amp resistant

More information

Strain: CC-118 sr-u-2-60

Insert: EcoRI-BamHI (2.0 kb), subclone of Bam 11/12

Genes present: 16S rRNA (5′ end)

Vector: pUC8

Bacterial host strain: TG-1

Selectable marker: amp-r, white on X-gal

Origin: Boynton/Gillham lab, 1990

The sr-u-2-60 mutation is an A->C change at base 474 in C. reinhardtii 16S rRNA gene that produces streptomycin resistance.
See Harris et al., Genetics 123, 281 (1989).

Harris EH, Burkhart BD, Gillham NW, Boynton JE (1989) Antibiotic resistance mutations in the chloroplast 16S and 23S rRNA genes of Chlamydomonas reinhardtii: correlation of genetic and physical maps of the chloroplast genome. Genetics 123:281-292

P-198 cpDNA EcoRI-BamHI (2.0 kb) sr-u-2-60

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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