P-239 cpDNA KpnI-HindIII (3.3 kb) atpB disrupted

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA KpnI-HindIII (3.3 kb) in pUC18, from a cw15 strain equivalent to CC-400; atpB disrupted with YEp24

host strain: JM83
amp resistant

 

More information

Strain: Rochaix cw15

Insert: KpnI-HindIII (3.3 kb)

Genes present: atpB (disrupted)

Vector: pUC18

Bacterial host strain: JM83

Selectable marker: SELECTION: amp-r, white on X-gal

Origin: Scott Newman in Boynton/Gillham lab, 1990

P-239

The insert contains a 0.48 kb PstI fragment from yeast plasmid YEp24 (P-238) cloned into the unique PstI site of the 2.8 kb KpnI-HindIII fragment (P-237), disrupting the atpB gene. P239A and B contain this Pst fragment inserted in opposite orientations. The plasmid was used in transformation experiments by Newman et al. (1991) Mol. Gen. Genet. 230, 65-74). See Figure 1 of that paper.

Newman SM, Gillham NW, Harris EH, Johnson AM, Boynton JE (1991) Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii. Mol Gen Genet 230:65-74