P-259 cpDNA XhoI 8 (11.0 kb) er-u-1a

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA XhoI 8 (11.0 kb) in BSKS+; contains psbA, 5S, and 3′ end of 23S with er-u-1a mutation from CC-64

host strain: JM109
amp resistant

 

More information

Strain: CC-64 er-u-1a mt+

Insert: XhoI 8 (11.0 kb)

Genes present: psbA, 5S rRNA, 23S rRNA (small exon)

Vector: BSKS+

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Amnon Lers in Boynton/Gillham lab, 1990

P-259

This map is from the original file for the plasmid and was drawn before the complete sequence was known.

The er-u-1a mutation is a C->T change at bp 2622 in C. reinhardtii 23S rRNA gene (Lemieux numbering, equivalent to E. coli 2611), which confers erythromycin resistance in Chlamydomonas. The insert was cloned in the XhoI site of the BSKS+ polylinker in both orientations. In P-259A, the psbA gene direction is from the KS SacI site towards the KS KpnI site; in P-259B, the psbA direction is from the KS KpnI site towards the KS SacI site.