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P-270 cpDNA BamHI-EcoRI (4.48 kb) rbcL disrupted

Name: P-270 cpDNA BamHI-EcoRI (4.48 kb) rbcL disrupted
SKU: P-270
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1990

Chloroplast DNA BamHI-EcoRI (4.48 kb) in pUC18 with Pst site deleted, rbcL gene disrupted with YEp24. The DNA was obtained from CC-125 wild type.

host strain: JM83
amp resistant

More information

Strain: CC-125 wild type mt+

Insert: BamHI-EcoRI (4.48 kb)

Genes present: rbcL, disrupted with 0.48 kb Pst I fragment from yeast plasmid YEp24

Vector: pUC18 with PstI site deleted

Bacterial host strain: JM83

Selectable marker: amp-r

Origin: Scott Newman in Boynton/Gillham lab, 1990

The insert contains the 0.48 kb Pst I fragment from yeast plasmid YEp24 (P-238), cloned into unique Pst site of a 4 kb Bam-Eco fragment of cpDNA, disrupting the rbcL gene. This construct was used in transformation experiments by Newman et al. (1991) Mol. Gen. Genet. 230, 65-74.

P-270 cpDNA BamHI-EcoRI (4.48 kb) rbcL disrupted

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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