P-361 cpDNA EcoRI-(XhoI) (3.6 kb) psbA DCMU4

$30.00

Boynton-Gillham laboratory, Duke University, 1991

Chloroplast DNA EcoRI-(XhoI) (3.6 kb) in pUC18; psbA exon 5 with DCMU4 mutation, 5S and 23S 3′ end; Kpn deletion between psbA and 5S

host strain: JM109
amp resistant

 

More information

Strain: CC-2534 DCMU-4 er-u-1a

Insert: EcoRI-(XhoI) (3.6 kb)

Genes present: psbA – 23S rRNA, Kpn deletion

Vector: pUC18

Bacterial host strain: JM109

Selectable marker: amp-r

Origin: Scott Newman in Boynton/Gillham lab, 1991

P-361

Insert = 2.2 kb Eco-Kpn fragment from P-322 containing 5th exon of psbA, ligated to 1.4 kb Kpn-Sal fragment from P-14 (Sal site located in BSKS polylinker) containing 5S rRNA gene and small exon of 23S rRNA gene. Resulting construct has Kpn fragments between psbA and 5S rRNA deleted as in P-320, but has wild type (erythromycin sensitive) 23S gene derived from P-14.

The DCMU-4 mutation in 5th exon of C. reinhardtii psbA gene causes a ser -> ala change at aa 264 of D1 protein, conferring resistance to DCMU and atrazine.