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P-377 cpDNA KpnI-BamHI (2.6 kb) er-u-1a
$30.00
Boynton-Gillham laboratory, Duke University, 1991
Chloroplast DNA KpnI-BamHI (2.6 kb) in pUC18; 5S and 3′ end of 23S with er-u-1a mutation
host strain: JM109
amp resistant
More information
Strain: CC-64 er-u-1a
Insert: KpnI-BamHI (2.6 kb)
Genes present: 23S rRNA, 3′ end
Vector: pUC18
Bacterial host strain: JM109
Selectable marker: amp-r
Origin: Scott Newman in Boynton/Gillham lab, 1991
This is a subclone of P-321, which was a ligation of sequences from CC-2534 and CC-64. The fragment remaining in this subclone derives mainly from CC-64.
The er-u-1a mutation is a C > T change at bp 2622 in C. reinhardtii 23S rRNA (Lemieux numbering, equivalent to E. coli 2611), conferring resistance to erythromycin.