* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
P-386 cpDNA BamHI 11/12 spr-u-1-27-3
$30.00
Boynton-Gillham laboratory, Duke University, 1991
Chloroplast DNA BamHI 11/12 in a pUC18 deletion plasmid; contains 16S and 5′ end of 23S, spr-u-1-27-3 mutation in 23S rRNA, from CC-105
host strain: JM83
amp resistant
More information
Strain: CC-105 spr-u-1-27-3 mt+
Insert: Bam 11/12 (7.0 kb)
Genes present: 16S and 23S rRNA
Vector: pUC18 with Nde, Eco and Pst sites deleted
Bacterial host strain: JM83
Selectable marker: amp-r
Origin: Charles Hauser in Boynton/Gillham lab, 1991
The mutation in spr-u-1-27-3 is a G -> A change at bp 1125 in the 16S chloroplast ribosomal RNA.