P-657 cpDNA atpB, with rps4 5′: aadA : rbcL 3′

$30.00

Boynton-Gillham laboratory, Duke University, 1997

E. coli aadA gene flanked by C. reinhardtii rps4 5′ and rbcL 3′; inserted downstream of the atpB gene but in the opposite orientation; used to transform an atpB deletion mutant. Eco-Bam (7.4 kb) in
pUC8.

host strain: XL1-Blue
amp resistant


Fargo DC, Zhang M, Gillham NW, Boynton JE (1998) Shine-Dalgarno-like sequences are not required for translation of chloroplast mRNAs in Chlamydomonas reinhardtii chloroplasts or in Escherichia coli. Mol Gen Genet 257:271-282